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Single Molecule Imaging of Fluorescently Labeled Proteins on Metal by Surface Plasmons in Aqueous Solution

Hiroaki Yokota, Kiwamu Saito, and Toshio Yanagida
Phys. Rev. Lett. 80, 4606 – Published 18 May 1998
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Abstract

We report the first real time imaging of single fluorophores attached to protein molecules on metal surfaces in aqueous solution using surface plasmon resonance fluorescence microscopy. The fluorescence was enhanced by the surface plasmons as theoretically predicted for gold and silver. Active movement of single molecules of the fluorescently labeled motor protein, coupled to the ATPase reaction, was observed on the surfaces of gold and aluminum. This microscopy should prove a powerful tool to directly detect single molecule processes in biomolecule systems organized on a metal surface.

  • Received 18 August 1997

DOI:https://doi.org/10.1103/PhysRevLett.80.4606

©1998 American Physical Society

Authors & Affiliations

Hiroaki Yokota1, Kiwamu Saito2, and Toshio Yanagida1,2,3,*

  • 1Department of Biophysical Engineering, Osaka University, 1-3 Machikaneyama, Toyonaka, Osaka 560-8531, Japan
  • 2Yanagida Biomotron Project, ERATO, JST, 2-4-14 Senba-Higashi, Mino, Osaka 562-0035, Japan
  • 3Department of Physiology, Osaka University Medical School, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan

  • *To whom correspondence should be addressed: Department of Physiology, Osaka University Medical School, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan. Electronic address: yanagida@phys1.med.osaka-u.ac.jp

See Also

Motor Proteins Move on Metal

Phys. Rev. Focus 1, 15 (1998)

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Issue

Vol. 80, Iss. 20 — 18 May 1998

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