Abstract
We present a proof-of-concept three-dimensional reconstruction of the giant mimivirus particle from experimentally measured diffraction patterns from an x-ray free-electron laser. Three-dimensional imaging requires the assembly of many two-dimensional patterns into an internally consistent Fourier volume. Since each particle is randomly oriented when exposed to the x-ray pulse, relative orientations have to be retrieved from the diffraction data alone. We achieve this with a modified version of the expand, maximize and compress algorithm and validate our result using new methods.
- Received 3 October 2014
DOI:https://doi.org/10.1103/PhysRevLett.114.098102
© 2015 American Physical Society
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X-Ray Imaging of a Single Virus in 3D
Published 2 March 2015
An x-ray laser has imaged the three-dimensional structure of the mimivirus by combining hundreds of measurements on single virus particles.
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