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Three-Dimensional Reconstruction of the Giant Mimivirus Particle with an X-Ray Free-Electron Laser

Tomas Ekeberg et al.
Phys. Rev. Lett. 114, 098102 – Published 2 March 2015
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Abstract

We present a proof-of-concept three-dimensional reconstruction of the giant mimivirus particle from experimentally measured diffraction patterns from an x-ray free-electron laser. Three-dimensional imaging requires the assembly of many two-dimensional patterns into an internally consistent Fourier volume. Since each particle is randomly oriented when exposed to the x-ray pulse, relative orientations have to be retrieved from the diffraction data alone. We achieve this with a modified version of the expand, maximize and compress algorithm and validate our result using new methods.

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  • Received 3 October 2014

DOI:https://doi.org/10.1103/PhysRevLett.114.098102

© 2015 American Physical Society

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X-Ray Imaging of a Single Virus in 3D

Published 2 March 2015

An x-ray laser has imaged the three-dimensional structure of the mimivirus by combining hundreds of measurements on single virus particles.

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Vol. 114, Iss. 9 — 6 March 2015

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