Abstract
Stained end-grafted DNA molecules about long are scraped away and stretched out by the spreading front of a bioadhesive vesicle. Tethered biotin ligands bind the vesicle bilayer to a streptavidin substrate, stapling the DNAs into frozen confinement paths. Image analysis of the stapled DNA gives access, within optical resolution, to the local stretching values of individual DNA molecules swept by the spreading front, and provides evidence of self-entanglements.
- Received 17 February 2010
DOI:https://doi.org/10.1103/PhysRevLett.105.088101
© 2010 The American Physical Society