• Open Access

Single-shot femtosecond x-ray diffraction from randomly oriented ellipsoidal nanoparticles

M. J. Bogan, S. Boutet, A. Barty, W. H. Benner, M. Frank, L. Lomb, R. Shoeman, D. Starodub, M. M. Seibert, S. P. Hau-Riege, B. Woods, P. Decorwin-Martin, S. Bajt, J. Schulz, U. Rohner, B. Iwan, N. Timneanu, S. Marchesini, I. Schlichting, J. Hajdu, and H. N. Chapman
Phys. Rev. ST Accel. Beams 13, 094701 – Published 14 September 2010

Abstract

Coherent diffractive imaging of single particles using the single-shot “diffract and destroy” approach with an x-ray free electron laser (FEL) was recently demonstrated. A high-resolution low-noise coherent diffraction pattern, representative of the object before it turns into a plasma and explodes, results from the interaction of the FEL with the particle. Iterative phase retrieval algorithms are used to reconstruct two-dimensional projection images of the object from the recorded intensities alone. Here we describe the first single-shot diffraction data set that mimics the data proposed for obtaining 3D structure from identical particles. Ellipsoidal iron oxide nanoparticles (250nm×50nm) were aerosolized and injected through an aerodynamic lens stack into a soft x-ray FEL. Particle orientation was not controlled with this injection method. We observed that, at the instant the x-ray pulse interacts with the particle, a snapshot of the particle’s orientation is encoded in the diffraction pattern. The results give credence to one of the technical concepts of imaging individual nanometer and subnanometer-sized objects such as single molecules or larger clusters of molecules using hard x-ray FELs and will be used to help develop robust algorithms for determining particle orientations and 3D structure.

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  • Received 30 November 2009

DOI:https://doi.org/10.1103/PhysRevSTAB.13.094701

This article is available under the terms of the Creative Commons Attribution 3.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI.

© 2010 The American Physical Society

Authors & Affiliations

M. J. Bogan1,*, S. Boutet2, A. Barty3,4, W. H. Benner3, M. Frank3, L. Lomb5, R. Shoeman5, D. Starodub1, M. M. Seibert6, S. P. Hau-Riege3, B. Woods3, P. Decorwin-Martin1, S. Bajt7, J. Schulz4, U. Rohner3,8, B. Iwan6, N. Timneanu6, S. Marchesini9, I. Schlichting5, J. Hajdu6, and H. N. Chapman4,10

  • 1Stanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA
  • 2LCLS, SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA
  • 3Lawrence Livermore National Laboratory, Livermore, California 94550, USA
  • 4Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
  • 5Max Planck Institute for Medical Research, Heidelberg, Germany Germany, and Max Planck Advanced Study Group, Center for Free-Electron Laser Science, DESY, Hamburg, Germany
  • 6Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Uppsala, Sweden
  • 7Photon Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
  • 8TOFWERK AG, Thun, Switzerland
  • 9Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA
  • 10University of Hamburg, Luruper Chaussee 149, 22761 Hamburg, Germany

  • *mbogan@slac.stanford.edu

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Vol. 13, Iss. 9 — September 2010

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