Diffusivelike Motions in a Solvent-Free Protein-Polymer Hybrid

Giorgio Schirò, Yann Fichou, Alex P. S. Brogan, Richard Sessions, Wiebke Lohstroh, Michaela Zamponi, Gerald J. Schneider, François-Xavier Gallat, Alessandro Paciaroni, Douglas J. Tobias, Adam Perriman, and Martin Weik
Phys. Rev. Lett. 126, 088102 – Published 25 February 2021
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Abstract

The interaction between proteins and hydration water stabilizes protein structure and promotes functional dynamics, with water translational motions enabling protein flexibility. Engineered solvent-free protein-polymer hybrids have been shown to preserve protein structure, function, and dynamics. Here, we used neutron scattering, protein and polymer perdeuteration, and molecular dynamics simulations to explore how a polymer dynamically replaces water. Even though relaxation rates and vibrational properties are strongly modified in polymer coated compared to hydrated proteins, liquidlike polymer dynamics appear to plasticize the conjugated protein in a qualitatively similar way as do hydration-water translational motions.

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  • Received 4 July 2020
  • Accepted 11 January 2021

DOI:https://doi.org/10.1103/PhysRevLett.126.088102

© 2021 American Physical Society

Physics Subject Headings (PhySH)

Physics of Living SystemsPolymers & Soft Matter

Authors & Affiliations

Giorgio Schirò1,*,†, Yann Fichou2,*,‡, Alex P. S. Brogan3, Richard Sessions4, Wiebke Lohstroh5, Michaela Zamponi6, Gerald J. Schneider7, François-Xavier Gallat1, Alessandro Paciaroni8, Douglas J. Tobias9, Adam Perriman10, and Martin Weik1,§

  • 1Université Grenoble Alpes, CEA, CNRS, Institut de Biologie Structurale, F-38000 Grenoble, France
  • 2CNRS, Chemistry and Biology of Membranes and Nanoobjects (CBMN) UMR 5348, Institut Europeen de Chimie et Biologie (IECB), University of Bordeaux, 33600 Pessac, France
  • 3School of Biochemistry, Medical Sciences Building, University Walk, Bristol BS8 1TD, United Kingdom
  • 4Department of Chemistry, King’s College London, Britannia House, London SE1 1DB, United Kingdom
  • 5Heinz Maier-Leibnitz Zentrum, Technische Universität München, Garching, Germany
  • 6Forschungszentrum Jülich GmbH, Jülich Centre for Neutron Science at Heinz Maier-Leibnitz Zentrum, Garching, Germany
  • 7Department of Chemistry and Department of Physics & Astronomy, Louisiana State University, Baton Rouge, Louisiana 70803, USA
  • 8Dipartimento di Fisica e Geologia, Università degli Studi di Perugia, 06123 Perugia, Italy
  • 9Department of Chemistry, University of California, Irvine, California, CA 92697, USA
  • 10School of Cellular and Molecular Medicine, University of Bristol, Bristol BS8 1TL, United Kingdom

  • *These authors contributed equally to the work.
  • giorgio.schiro@ibs.fr
  • y.fichou@iecb.u-bordeaux.fr
  • §martin.weik@ibs.fr

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Vol. 126, Iss. 8 — 26 February 2021

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