Abstract
Flexibility, or softness, is crucial for protein function and consists of a conformational component, involving jumps between potential wells, and an elastic component, involving fluctuations within the wells. Combining molecular dynamics simulation with incoherent neutron scattering and light scattering measurements on green fluorescent protein, we reveal a relationship between the intrawell fluctuations and elastic moduli of the protein. This finding leads to a simple means of experimentally separating the conformational from the elastic atomic displacements.
- Received 1 November 2012
- Corrected 29 January 2013
DOI:https://doi.org/10.1103/PhysRevLett.110.028104
© 2013 American Physical Society
Corrections
29 January 2013
Erratum
Publisher’s Note: Elastic and Conformational Softness of a Globular Protein [Phys. Rev. Lett. 110, 028104 (2013)]
Liang Hong, Dennis C. Glass, Jonathan D. Nickels, Stefania Perticaroli, Zheng Yi, Madhusudan Tyagi, Hugh O’Neill, Qiu Zhang, Alexei P. Sokolov, and Jeremy C. Smith
Phys. Rev. Lett. 110, 069901 (2013)
Synopsis
Classifying Protein Wiggles
Published 10 January 2013
Two different mechanisms give a protein its required flexibility.
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