Characterizing Concentrated, Multiply Scattering, and Actively Driven Fluorescent Systems with Confocal Differential Dynamic Microscopy

Peter J. Lu (陸述義), Fabio Giavazzi, Thomas E. Angelini, Emanuela Zaccarelli, Frank Jargstorff, Andrew B. Schofield, James N. Wilking, Mark B. Romanowsky, David A. Weitz, and Roberto Cerbino
Phys. Rev. Lett. 108, 218103 – Published 22 May 2012
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Abstract

We introduce confocal differential dynamic microscopy (ConDDM), a new technique yielding information comparable to that given by light scattering but in dense, opaque, fluorescent samples of micron-sized objects that cannot be probed easily with other existing techniques. We measure the correct wave vector q-dependent structure and hydrodynamic factors of concentrated hard-sphere-like colloids. We characterize concentrated swimming bacteria, observing ballistic motion in the bulk and a new compressed-exponential scaling of dynamics, and determine the velocity distribution; by contrast, near the coverslip, dynamics scale differently, suggesting that bacterial motion near surfaces fundamentally differs from that of freely swimming organisms.

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  • Received 3 June 2010

DOI:https://doi.org/10.1103/PhysRevLett.108.218103

© 2012 American Physical Society

Authors & Affiliations

Peter J. Lu (陸述義)1, Fabio Giavazzi2, Thomas E. Angelini1, Emanuela Zaccarelli3, Frank Jargstorff4, Andrew B. Schofield5, James N. Wilking1, Mark B. Romanowsky1, David A. Weitz1, and Roberto Cerbino2

  • 1Department of Physics and SEAS, Harvard University, Cambridge, Massachusetts 02138, USA
  • 2Dipartimento di Chimica, Biochimica e Biotecnologie per la Medicina, Universita degli Studi di Milano, I-20090, Italy
  • 3CNR-ISC and Dipartimento di Fisica, Universita di Roma La Sapienza, I-00185 Rome, Italy
  • 4NVIDIA, Santa Clara, California 95050, USA
  • 5Department of Physics, University of Edinburgh, Edinburgh EH9 3JZ, United Kingdom

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Issue

Vol. 108, Iss. 21 — 25 May 2012

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