Cryogenic X-Ray Diffraction Microscopy for Biological Samples

Enju Lima, Lutz Wiegart, Petra Pernot, Malcolm Howells, Joanna Timmins, Federico Zontone, and Anders Madsen
Phys. Rev. Lett. 103, 198102 – Published 5 November 2009

Abstract

X-ray diffraction microscopy (XDM) is well suited for nondestructive, high-resolution biological imaging, especially for thick samples, with the high penetration power of x rays and without limitations imposed by a lens. We developed nonvacuum, cryogenic (cryo-) XDM with hard x rays at 8 keV and report the first frozen-hydrated imaging by XDM. By preserving samples in amorphous ice, the risk of artifacts associated with dehydration or chemical fixation is avoided, ensuring the imaging condition closest to their natural state. The reconstruction shows internal structures of intact D. radiodurans bacteria in their natural contrast.

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  • Received 1 June 2009

DOI:https://doi.org/10.1103/PhysRevLett.103.198102

©2009 American Physical Society

Authors & Affiliations

Enju Lima*, Lutz Wiegart, Petra Pernot, Malcolm Howells, Joanna Timmins, Federico Zontone, and Anders Madsen

  • European Synchrotron Radiation Facility, B.P. 220, F-38043 Grenoble, France

  • *Corresponding author. elima@bnl.gov Present address: Brookhaven National Laboratory, Upton, NY 11973, USA.
  • amadsen@esrf.fr

See Also

Soft X-Ray Diffraction Microscopy of a Frozen Hydrated Yeast Cell

Xiaojing Huang, Johanna Nelson, Janos Kirz, Enju Lima, Stefano Marchesini, Huijie Miao, Aaron M. Neiman, David Shapiro, Jan Steinbrener, Andrew Stewart, Joshua J. Turner, and Chris Jacobsen
Phys. Rev. Lett. 103, 198101 (2009)

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Vol. 103, Iss. 19 — 6 November 2009

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