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Quantitative Imaging of Single, Unstained Viruses with Coherent X Rays

Changyong Song, Huaidong Jiang, Adrian Mancuso, Bagrat Amirbekian, Li Peng, Ren Sun, Sanket S. Shah, Z. Hong Zhou, Tetsuya Ishikawa, and Jianwei Miao
Phys. Rev. Lett. 101, 158101 – Published 7 October 2008

Abstract

We report the recording and reconstruction of x-ray diffraction patterns from single, unstained viruses, for the first time. By separating the diffraction pattern of the virus particles from that of their surroundings, we performed quantitative and high-contrast imaging of a single virion. The structure of the viral capsid inside a virion was visualized. This work opens the door for quantitative x-ray imaging of a broad range of specimens from protein machineries and viruses to cellular organelles. Moreover, our experiment is directly transferable to the use of x-ray free electron lasers, and represents an experimental milestone towards the x-ray imaging of large protein complexes.

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  • Received 8 July 2008

DOI:https://doi.org/10.1103/PhysRevLett.101.158101

©2008 American Physical Society

Authors & Affiliations

Changyong Song1, Huaidong Jiang1, Adrian Mancuso1, Bagrat Amirbekian1, Li Peng2, Ren Sun2, Sanket S. Shah3, Z. Hong Zhou4, Tetsuya Ishikawa5, and Jianwei Miao1,*

  • 1Department of Physics and Astronomy, University of California, Los Angeles, California 90095, USA
  • 2Department of Molecular and Medical Pharmacology, University of California, Los Angeles, California 90095, USA
  • 3Department of Pathology and Laboratory Medicine, University of Texas-Houston Medical School, Texas 77030, USA
  • 4Department of Microbiology, Immunology & Molecular Genetics, University of California, Los Angeles, California 90095, USA
  • 5RIKEN SPring-8 Center, 1-1-1, Kouto, Sayo, Hyogo 679-5148, Japan

  • *Corresponding author. miao@physics.ucla.edu.

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Issue

Vol. 101, Iss. 15 — 10 October 2008

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