Spectroscopy of Single Hemoglobin Molecules by Surface Enhanced Raman Scattering

Hongxing Xu, Erik J. Bjerneld, Mikael Käll, and Lars Börjesson
Phys. Rev. Lett. 83, 4357 – Published 22 November 1999
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Abstract

We demonstrate the detection of molecular vibrations in single hemoglobin (Hb) protein molecules attached to isolated and immobilized silver nanoparticles by surface enhanced Raman scattering (SERS). A comparison between calculation and experiment indicates that electromagnetic field effects dominate the surface enhancement, and that single molecule Hb SERS is possible only for molecules situated between Ag particles. The vibrational spectra exhibit temporal fluctuations of unknown origin which appear to be characteristic of the single molecule detection limit.

  • Received 22 January 1999

DOI:https://doi.org/10.1103/PhysRevLett.83.4357

©1999 American Physical Society

Authors & Affiliations

Hongxing Xu, Erik J. Bjerneld, Mikael Käll*, and Lars Börjesson

  • Department of Applied Physics, Chalmers University of Technology, S-412 96 Göteborg, Sweden

  • *Corresponding author.Email address: kall@fy.chalmers.se

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Issue

Vol. 83, Iss. 21 — 22 November 1999

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