Observation of a Rectangular Columnar Phase in Condensed Lamellar Cationic Lipid-DNA Complexes

F. Artzner, R. Zantl, G. Rapp, and J. O. Rädler
Phys. Rev. Lett. 81, 5015 – Published 30 November 1998
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Abstract

We report on a synchrotron x-ray diffraction study of saturated cationic lipid complexed with DNA. In the lipid gel phase Lβc the complexes exhibit Bragg reflections due to lamellar lipid bilayer stacking and three nonintegral peaks in agreement with an intercalated centered rectangular columnar superlattice of DNA. The diffuse broadening of the DNA peaks is caused by in-plane translational disorder of the DNA strands and is approximated by Lorentzians with positional correlation lengths of ξy250 out of plane and ξx>2000 in plane. We interpret our results in terms of a system of interacting two-dimensional smectic layers.

  • Received 15 May 1998

DOI:https://doi.org/10.1103/PhysRevLett.81.5015

©1998 American Physical Society

Authors & Affiliations

F. Artzner1,*, R. Zantl1, G. Rapp2, and J. O. Rädler1,†

  • 1Lehrstuhl für Biophysik, E22, Technische Universität München, James-Franck-Strasse 1, D-85748 Garching, Germany
  • 2European Molecular Biology Laboratory, EMBL c/o DESY, Notkestrasse 85, D-22603 Hamburg, Germany

  • *Permanent address: Equipe Physico-chimie des systèmes Polyphasés, CNRS URA 1218, 5, rue J. B. Clément F-92296 Châtenay-Malabry, France.
  • Email address: raedler@physik.tu-muenchen.de

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Vol. 81, Iss. 22 — 30 November 1998

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