Drug Binding to DNA: Observation of the Drug-DNA Hydrogen-Bond-Stretching Modes of Netropsin Bound to DNA via Raman Spectroscopy

S. A. Lee; A. Rupprecht; Y. Z. Chen

doi:10.1103/PhysRevLett.80.2241

Drug Binding to DNA: Observation of the Drug-DNA Hydrogen-Bond-Stretching Modes of Netropsin Bound to DNA via Raman Spectroscopy

S. A. Lee, A. Rupprecht, and Y. Z. Chen

Phys. Rev. Lett. 80, 2241 – Published 9 March 1998

Abstract

Raman scattering and normal mode calculations have been used to study the low frequency vibrations of NaDNA, netropsin, and the NaDNA·netropsin complex. Vibrational modes at about 60 and $180 {cm}^{- 1}$ are observed from the DNA·netropsin complex but not from either pure DNA or pure netropsin. Theoretical calculations show that these are stretching modes of the hydrogen bonds connecting netropsin to DNA. Identification of these modes in this and other systems may facilitate the study of biomolecule·ligand binding dynamics.

Received 1 October 1997

DOI:https://doi.org/10.1103/PhysRevLett.80.2241

Authors & Affiliations

S. A. Lee¹, A. Rupprecht², and Y. Z. Chen^1,3

¹Department of Physics & Astronomy, University of Toledo, Toledo, Ohio 43606
²Division of Physical Chemistry, Arrhenius Laboratory, University of Stockholm, S-106 91, Stockholm, Sweden
³Department of Computational Sciences, National University of Singapore, Singapore 119260