Mechanisms and Dynamics of Protein Clustering on a Solid Surface

P. A. Mulheran, D. Pellenc, R. A. Bennett, R. J. Green, and M. Sperrin
Phys. Rev. Lett. 100, 068102 – Published 12 February 2008

Abstract

A methodology for discovering the mechanisms and dynamics of protein clustering on solid surfaces is presented. In situ atomic force microscopy images are quantitatively compared to Monte Carlo simulations using cluster statistics to differentiate various models. We study lysozyme adsorption on mica as a model system and find that all surface-supported clusters are mobile, not just the monomers, with diffusion constant inversely related to cluster size. The surface monomer diffusion constant is measured to be D19×1016cm2s1, such a low value being difficult to measure using other techniques.

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  • Received 13 February 2007

DOI:https://doi.org/10.1103/PhysRevLett.100.068102

©2008 American Physical Society

Authors & Affiliations

P. A. Mulheran1, D. Pellenc2, R. A. Bennett2, R. J. Green3, and M. Sperrin4

  • 1Department of Chemical and Process Engineering, University of Strathclyde, James Weir Building, 75 Montrose Street, Glasgow G1 1XJ, United Kingdom
  • 2Department of Physics, University of Reading, Whiteknights, Reading RG6 6AF, United Kingdom
  • 3School of Pharmacy, University of Reading, Whiteknights, Reading RG6 6AF, United Kingdom
  • 4Medical Physics and Clinical Engineering, Royal Berkshire Hospital, London Road, Reading RG1 5AN, United Kingdom

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Issue

Vol. 100, Iss. 6 — 15 February 2008

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