DNA electrophoresis in a monodisperse porous medium

L. Meistermann and B. Tinland
Phys. Rev. E 62, 4014 – Published 1 September 2000
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Abstract

Electrophoresis of DNA migrating in an ordered matrix is studied and compared with classical agarose gel electrophoresis. A well-defined migration medium is obtained by crystallization of monodisperse silica spheres. Electrophoretic mobility of DNA is measured with fluorescence recovery after photobleaching experiments. The main result is that, as it was the case for gel electrophoresis, diffusion and electrophoretic mobility of DNA in such a medium are well described with reptation theories.

  • Received 5 October 1999

DOI:https://doi.org/10.1103/PhysRevE.62.4014

©2000 American Physical Society

Authors & Affiliations

L. Meistermann and B. Tinland

  • Institut Charles Sadron, CNRS, 6 rue Boussingault, 67083 Strasbourg, France

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Issue

Vol. 62, Iss. 3 — September 2000

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