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Detection and identification of a single DNA base molecule using surface-enhanced Raman scattering (SERS)

Katrin Kneipp, Harald Kneipp, V. Bhaskaran Kartha, Ramasamy Manoharan, Geurt Deinum, Irving Itzkan, Ramachandra R. Dasari, and Michael S. Feld
Phys. Rev. E 57, R6281(R) – Published 1 June 1998
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Abstract

Nonresonant Raman cross sections of 1016cm2 per molecule are shown to be related to surface-enhanced Raman scattering (SERS) on colloidal silver clusters at near-infrared (NIR) excitation. The enhancement is found to be independent of cluster sizes between 100 nm and 20 μm. These experimental findings demonstrate that NIR SERS on colloidal silver clusters is an excellent technique for single molecule detection that is applicable for a broad range of molecules including “colorless” biomolecules, for example nucleotides in DNA sequencing. As an example, we present the detection of a single adenine molecule without any labeling based on its intrinsic surface-enhanced Raman scattering.

  • Received 4 November 1997

DOI:https://doi.org/10.1103/PhysRevE.57.R6281

©1998 American Physical Society

Authors & Affiliations

Katrin Kneipp*, Harald Kneipp, V. Bhaskaran Kartha, Ramasamy Manoharan, Geurt Deinum, Irving Itzkan, Ramachandra R. Dasari, and Michael S. Feld

  • G. R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139

  • *Present address: Physics Department, Technical University Berlin, D-10623 Berlin, Germany.

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Vol. 57, Iss. 6 — June 1998

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