Vibrational dynamics of wet-spun films of the NaDNA-netropsin complex: A Raman and infrared study

Raman spectroscopy and infrared absorption have been used to study the vibrational dynamics of wet-spun films of the NaDNA-netropsin (NaDNA-net) complex as well as netropsin and NaDNA. The NaDNA-net complex is found to have a substantially different vibrational spectrum from either netropsin or NaDNA. The films of NaDNA-net appear to be more crystalline than the films of NaDNA alone. The formation of the NaDNA-net complex strongly favors the B conformation of the double helix. These vibrational data provide useful information for the understanding of the nature of the interaction of DNA with the antiviral, antitumor antibiotic netropsin.